期刊
MOLECULAR MEDICINE REPORTS
卷 5, 期 1, 页码 211-216出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2011.606
关键词
yessotoxin; apoptosis; HL7702 cells; mitochondrial transmembrane potential; calcium level
资金
- Ministry of Science and Technology of the People's Republic of China [2007DFA30710]
- First Institute of Oceanography, SOA [2011T07]
The marine toxin yessotoxin (YTX) is found in numerous aquatic environments and poses a potential threat to the shellfish industry and to public health. We analyzed the toxicity of YTX on HL7702 human liver cells using optical microscopy, Hoechst 33342 chromatin staining, DNA gel electrophoresis, rhodamine 123 staining and calcium-sensitive laser scanning confocal microscopy. The results demonstrated that YTX induced the usual hallmarks of apoptosis, including chromain condensation. DNA laddering, activity of caspase-3 deregulation and loss of mitochondrial membrane potential. Furthermore, YTX caused cytosolic calcium levels to increase in HL7702 cells. YTX may cause liver damage through hepatocyte apoptosis.
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