期刊
MOLECULAR IMMUNOLOGY
卷 58, 期 1, 页码 17-26出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2013.10.011
关键词
Ascaris suum; High molecular weight components; Immunoregulation; Dendritic Cells; Toll like receptor 2 (TLR2); Toll like receptor 4 (TLR4)
资金
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2008/04201-2, 2011/23735-0]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- FAPESP [2007/56883-7, 2010/10393-1]
- CAPES
Components of high molecular-weight (PI) obtained from Ascaris suum extract down-regulate the Th1/Th2-related immune responses induced by ovalbumin (OVA)-immunization in mice. Furthermore, the PI down-modulates the ability of dendritic cells (DCs) to activate T lymphocytes by an IL-10-mediated mechanism. Here, we evaluated the role of toll like receptors 2 and 4 (TLR2 and 4) in the modulatory effect of PI on OVA-specific immune response and the PI interference on DC full activation. An inhibition of OVA-specific cellular and humoral responses were observed in wild type (WT) or in deficient in TLR2 (TLR2(-/-)) or 4 (TLR4(-/-)) mice immunized with OVA plus PI when compared with OVA-immunized mice. Low expression of class II MHC, CD40, CD80 and CD86 molecules was observed in lymph node (LN) cells from WT, TLR2(-/-) or TLR4(-/-) mice immunized with OVA plus PI compared with OVA-primed cells. We also verified that PI was able to modulate the activation of DCs derived from bone marrow of WT, TLR2(-/-) or TLR4(-/-) mice induced in vitro by agonists of TLRs, as observed by a decreased expression of class II MHC and costimulatory molecules and by low secretion of pro-inflammatory cytokines. Its effect was accompanied by IL-10 synthesis. In this sense, the modulatory effect of PI on specific-immune response and DC activation is independent of TLR2 or TLR4. (C) 2013 Elsevier Ltd. All rights reserved.
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