期刊
MOLECULAR IMMUNOLOGY
卷 46, 期 11-12, 页码 2200-2209出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2009.04.013
关键词
H2O2; Superoxide; Fc epsilon RI beta-chain ITAM; Store-operated Ca2+ channel
资金
- MEXT
- Nihon University
Mast cells and T cells produce reactive oxygen species (ROS) after stimulation with the high-affinity IgE receptor (Fc epsilon RI) and T cell receptor. A growing body of evidence suggests the existence of ROS-regulated intracellular and/or plasma membrane Ca2+ channels in these cells but their molecular entities remain to be identified. Here, we report that store-operated Ca2+ channel (SOC) activity is regulated by superoxide (O-2(center dot-)) and hydrogen peroxide (H2O2) in mast cells. MnTBaP (MR(III)tetrakis(4-benzoic acid)porphyrin) and ebselen (2-phenyl-1,2-benziso-selenazol-3(2H)-one) selectively blocked the generation of O-2(center dot-) and H2O2, respectively, in antigen-stimulated cells. The H2O2 generation was dependent on the Src family kinase (SFK) and phosphatidylinositol-3-kinase (PI3K) activities but independent of extracellular Ca2+, and the Fc epsilon RI beta-chain immunoreceptor tyrosine-based activation motif played an essential role. On the other hand, O-2(center dot-) generation was strictly dependent on extracellular Ca2+, but negatively regulated by the SFK and PI3K activities. Inhibition of O-2(center dot-) generation resulted in increased H2O2 generation and reduced SOC activity, although it had a minimal effect on endoplasmic reticulum Ca2+ store depletion. On the contrary, inhibition of H2O2 generation resulted in increased intracellular O-2(center dot-) generation and augmented SOC activity. The findings suggest that O-2(center dot-) and H2O2, which are generated by separate signaling pathways/sources, reciprocally regulate SOC activity in mast cells. Such generations of multiple oxidant species and their distinct roles in the regulation of SOC activity may facilitate the fine tuning of Ca2+ signaling in mast cells. (C) 2009 Elsevier Ltd. All rights reserved.
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