期刊
MOLECULAR IMAGING AND BIOLOGY
卷 16, 期 1, 页码 109-117出版社
SPRINGER
DOI: 10.1007/s11307-013-0664-5
关键词
Stroke; Neuroinflammation; Translocator protein 18 kDa (TSPO); Peripheral benzodiazepine receptor (PBR); Positron emission tomography (PET); [F-18]PBR06
资金
- Bio-X Interdisciplinary Initiatives Program (IIP) award from Stanford University
- Developmental Cancer Research Award (DCRA) in Translational Research from Stanford Cancer Institute
- NCI ICMIC [CA114747]
- American Heart Association [AHA-0835274]
- CIRM [RC1-0134]
- National Research Foundation of Korea
- Ministry of Education, Science and Technology, Korea [R31-10105, NRF-2012M3A9C6049796]
- Department of Radiation Oncology, Stanford University
The purpose of this study is to evaluate the 18 kDa translocator protein (TSPO) radioligand [F-18]N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline ([F-18]PBR06) as a positron emission tomography (PET) imaging biomarker of stroke-induced neuroinflammation in a rodent model. Stroke was induced by transient middle cerebral artery occlusion in Balb/c mice. Dynamic PET/CT imaging with displacement and preblocking using PK111195 was performed 3 days later. PET data were correlated with immunohistochemistry (IHC) for the activated microglial markers TSPO and CD68 and with autoradiography. [F-18]PBR06 accumulation peaked within the first 5 min postinjection, then decreased gradually, remaining significantly higher in infarct compared to noninfarct regions. Displacement or preblocking with PK11195 eliminated the difference in [F-18]PBR06 uptake between infarct and noninfarct regions. Autoradiography and IHC correlated well spatially with uptake on PET. [F-18]PBR06 PET specifically images TSPO in microglial neuroinflammation in a mouse model of stroke and shows promise for imaging and monitoring microglial activation/neuroinflammation in other disease models.
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