期刊
MOLECULAR IMAGING AND BIOLOGY
卷 13, 期 2, 页码 208-214出版社
SPRINGER
DOI: 10.1007/s11307-010-0355-4
关键词
Anesthesia; Motion artifact; Chick embryo; In vivo; In ovo; PET/CT; MicroCT; Model organism
资金
- BMBF [0314108]
The chick embryo in ovo is a well-accessible and economical in vivo model, but its use in molecular imaging has been limited because of motion artifacts on resulting images. The purpose of this study was to develop a method using narcotics to inhibit motility and to perform motion-artifact-free imaging of living chick embryos in ovo. Chick embryos in ovo were narcotized using three different narcotics: isoflurane, 2,2,2-tribromoethanol, and urethane/alpha-chloralose. Narcotized embryos were imaged using micro-computed tomography (microCT) at days 10-18 of incubation, and the resulting images were analyzed for reduction of motion artifacts. All three anesthetics could be used for anesthetizing living chick embryos in ovo thus allowing the acquisition of motion-artifact-free images. Our experiments revealed that isoflurane is the best-suited narcotic for single and repeated applications to image living chick embryos in ovo.
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