期刊
MOLECULAR IMAGING AND BIOLOGY
卷 12, 期 4, 页码 394-405出版社
SPRINGER
DOI: 10.1007/s11307-009-0288-y
关键词
Quantum dots; Lymph nodes; Near-infrared fluorescence imaging; Mass spectroscopy; Biodistribution
资金
- Institut National du Cancer (INCa)
- Ligue Contre le Cancer
- Lieue Nationale Contre le Cancer
- Region Lorraine
Purpose: This study compares fluorescence imaging to mass spectroscopy (inductively coupled plasma mass spectroscopy, ICP-MS) for detection of quantum dots (QDs) in sentinel lymph node (LN) mapping of breast cancer. Procedures: We study the accumulation of near-infrared-emitting QDs into regional LNs and their whole-body biodistribution in mice after subcutaneous injection, using in vivo fluorescence imaging and ex vivo elemental analysis by ICP-MS. Results: We show that the OD accumulation in regional LNs is detectable by fluorescence imaging as early as 5 min post-delivery. Their concentration reaches a maximum at 4 h then decreases over a 10-day observation period. These data are confirmed by ICP-MS. The QD uptake in other organs, assessed by ICP-MS, increases steadily over time; however, its overall level remains rather low. Conclusions: Fluorescence imaging can be used as a non-invasive alternative to ICP-MS to follow the QD accumulation kinetics into regional LNs.
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