期刊
MOLECULAR IMAGING AND BIOLOGY
卷 10, 期 5, 页码 264-270出版社
SPRINGER
DOI: 10.1007/s11307-008-0146-3
关键词
contrast agents; ultrasound; molecular imaging; perfluorocarbon; monocytes; leukocyte adhesion
Purpose:We investigated in vitro the potential of macrophages to act as targeted vehicle for ultrasound molecular imaging. Procedures: Murine bone marrow-derived macrophages (BMM), incubated for 3 h with different concentrations of perfluorohexane (PFH) emulsions, were monitored by microscopy, flow cytometry, and ultrasound. Effects of PFH loading on BMM adhesion molecule (PSGL-1, VLA-4, Mac-1, LFA-1) expression were analyzed by flow cytometry. Static adhesion of PFH loaded BMM to unstimulated and TNF-alpha stimulated b.End5 endothelial cells was assessed by microscopy. Results: Incubation of BMM with PFH emulsions resulted in dose-dependent uptake and increased echogenicity (max. 17 dB). Flow cytometry analyses revealed no down-regulation related to PFH loading of BMM adhesion molecule expression. Endothelial adhesion remained functional, even after 24 h, although PFH loading dose-dependently attenuated static adhesion. Conclusion: PFH loaded BMM may potentially serve as ultrasound contrast agent for noninvasive detection of atherogenic hotspots in arteries.
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