期刊
MOLECULAR HUMAN REPRODUCTION
卷 14, 期 8, 页码 431-444出版社
OXFORD UNIV PRESS
DOI: 10.1093/molehr/gan037
关键词
mitochondria; mitochondrial polarity; nitric oxide; cumulus cells; regulation of mitochondrial activity
Whether exogenous factors influenced the level of mitochondrial polarity (Delta Psi m) in the subplasmalemmal cytoplasm of the oocyte was investigated with denuded and cumulus-enclosed human and mouse oocytes between the germinal vesicle and metaphase II stage. Co-culture of denuded oocytes with cumulus masses or primary cumulus cell cultures demonstrated a 'proximity' effect with respect to the detectable level of Delta Psi m in the oocyte. The specificity and reversibility of this effect on subplasmalemmal mitochondria were shown by repeated repositioning between cellular and acellular regions, which sequentially down- or up-regulated Delta Psi m. Experimental studies with a nitric oxide (NO) donor and inhibitor of NO synthase indicate that NO produced by cumulus cells has a regulatory influence on Delta Psi m in the subplasmalemmal cytoplasm of the corresponding oocyte. Culture of denuded and cumulus-enclosed (intact) oocytes in low and high oxygen atmospheres suggests that competition between oxygen and NO at the mitochondrial level may regulate the level of Delta Psi m and maintain mitochondria homeostasis in the pre-ovulatory oocyte, with a shift to higher polarity occurring after ovulation. The role of exogenous influences on oocyte Delta Psi m is discussed with respect to the regulation of developmental processes in the oocyte and early embryo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据