期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 506, 期 -, 页码 279-286出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2014.11.010
关键词
Antibiotic resistance genes; Composting; Swine manure; Real-time PCR; Pollutant
资金
- National Natural Science Foundation of China [21377137, 20607024]
- CAS [XDA05020800]
The aim of this study was to compare the occurrence, abundance, and diversity of tetracycline resistance genes (tet) in agricultural soils after 6 years' application of fresh or composted swine manure. Soil samples were collected from fresh or composted manure-treated farmland at three depths (0-5 cm, 5-10 cm, and 10-20 cm). Nine classes of tet genes RetW, tetB(P), tet0, tetS, tetC, tetG, tetZ, tetL, and tetX] were detected; tetG, tetZ, tetL, and tetB(P) were predominant in the manure-treated soil. The abundances of tetB(P), tetW, tetC, and tet0 were reduced, while tetG and tetL were increased by fertilizing with composted versus fresh manure; thus, the total abundance of tet genes was not significantly reduced by compost manuring. tetG was the most abundant gene in manure-treated soil; the predominant tetG genotypes shared high homology with pathogenic bacteria. The tetC isolates were more diverse in soils treated with fresh versus composted manure, although the residual tet genes in composted manure remain a pollutant and produce a different influence on the tet gene resistome in field soil. (C) 2014 Elsevier B.V. All rights reserved.
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