期刊
MOLECULAR ENDOCRINOLOGY
卷 28, 期 4, 页码 512-524出版社
OXFORD UNIV PRESS INC
DOI: 10.1210/me.2013-1401
关键词
-
资金
- National Institutes of Health [HD065007, 1S10RR031586-01]
Maternal adiponectin (ADN) levels are inversely correlated with birth weight, and ADN infusion in pregnant mice down-regulates placental nutrient transporters and decreases fetal growth. In contrast to the insulin-sensitizing effects in adipose tissue and muscle, ADN inhibits insulin signaling in the placenta. However, the molecular mechanisms involved are unknown. We hypothesized that ADN inhibits insulin signaling and insulin-stimulated amino acid transport in primary human trophoblasts by peroxisome proliferator-activated receptor-alpha (PPAR alpha)-mediated ceramide synthesis. Primary human term trophoblast cells were treated with ADN and/or insulin. ADN increased the phosphorylation of p38 MAPK and PPAR alpha. ADN inhibited insulin signaling and insulin-stimulated amino acid transport. This effect was dependent on PPAR alpha, because activation of PPAR alpha with an agonist (GW7647) inhibited insulin signaling and function, whereas PPAR alpha small interfering RNA reversed the effects of ADN on the insulin response. ADN increased ceramide synthase expression and stimulated ceramide production. C-2-ceramide inhibited insulin signaling and function, whereas inhibition of ceramide synthase (with Fumonisin B1) reversed the effects of ADN on insulin signaling and amino acid transport. These findings are consistent with the model that maternal ADN limits fetal growth mediated by activation of placental PPAR alpha and ceramide synthesis, which inhibits placental insulin signaling and amino acid transport, resulting in reduced fetal nutrient availability.
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