4.7 Article

Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification

期刊

MOLECULAR ECOLOGY RESOURCES
卷 14, 期 5, 页码 1072-1079

出版社

WILEY
DOI: 10.1111/1755-0998.12241

关键词

InDels; Ion Torrent; marker design; next-generation sequencing; species delimitation; zooplankton

资金

  1. European Commission FP7 EURO-BASIN [264 933]
  2. Greenland Climate Research Centre [6505]
  3. Norwegian Research Council (HAVKYST) [216578]
  4. Medical Research Council [G0701127] Funding Source: researchfish
  5. Natural Environment Research Council [pml010004] Funding Source: researchfish
  6. MRC [G0701127] Funding Source: UKRI
  7. NERC [pml010004] Funding Source: UKRI

向作者/读者索取更多资源

Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids. Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae.

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