期刊
MOLECULAR ECOLOGY RESOURCES
卷 13, 期 4, 页码 740-745出版社
WILEY
DOI: 10.1111/1755-0998.12110
关键词
allele-specific gene expression; allelic imbalance; gene expression; mapping bias; RNA-seq
资金
- Austrian Science Funds (FWF) [P22834]
- Volkswagen Foundation
- Austrian Science Fund (FWF) [P 22834] Funding Source: researchfish
- Austrian Science Fund (FWF) [P22834] Funding Source: Austrian Science Fund (FWF)
Estimating differences in gene expression among alleles is of high interest for many areas in biology and medicine. Here, we present a user-friendly software tool, Allim, to estimate allele-specific gene expression. Because mapping bias is a major problem for reliable estimates of allele-specific gene expression using RNA-seq, Allim combines two different strategies to account for the mapping biases. In order to reduce the mapping bias, Allim first generates a polymorphism-aware reference genome that accounts for the sequence variation between the alleles. Then, a sequence-specific simulation tool estimates the residual mapping bias. Statistical tests for allelic imbalance are provided that can be used with the bias corrected RNA-seq data.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据