期刊
MOLECULAR ECOLOGY RESOURCES
卷 13, 期 6, 页码 1108-1115出版社
WILEY
DOI: 10.1111/1755-0998.12082
关键词
barcoding; identification; nematodes; primers
资金
- Government of Canada through Genome Canada
- Ontario Genomics Institute [2008-OGI-ICI-03]
- CONACyT Red del Codigo de Barras de la Vida, Mexico (MEXBOL)
- NSF [DEB01613802]
- PAPIIT-UNAM [IN-203911-3]
Although nematodes are one of the most diverse metazoan phyla, species identification through morphology is difficult. Several genetic markers have been used for their identification, but most do not provide species-level resolution in all groups, and those that do lack primer sets effective across the phylum, precluding high-throughput processing. This study describes a cocktail of three novel primer pairs that overcome this limitation by recovering cytochrome c oxidase I (COI) barcodes from diverse nematode lineages parasitic on vertebrates, including members of three orders and eight families. Its effectiveness across a broad range of nematodes enables high-throughput processing.
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