期刊
MOLECULAR ECOLOGY RESOURCES
卷 10, 期 6, 页码 942-945出版社
WILEY
DOI: 10.1111/j.1755-0998.2010.2839.x
关键词
Collembola; DNA barcoding; high-throughput; non-destructive DNA extraction; voucher retrieval
资金
- NSERC
- Genome Canada through the Ontario Genomics Institute
Here, we describe a simple method adapted for high-throughput protocols allowing voucher specimen recovery for Collembola and by extension for other soft-bodied small arthropods. A standard extraction protocol was tested to examine the effects of lysis duration (1, 2, 4, 12 h) on DNA concentration, amplification success and specimen condition. Good quality DNA was obtained after 1 h of lysis, while voucher condition was fine for up to 12 h. The lysis step substantially shortens the clearing process necessary for morphological examination.
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