期刊
MOLECULAR ECOLOGY RESOURCES
卷 11, 期 1, 页码 107-109出版社
WILEY
DOI: 10.1111/j.1755-0998.2010.02909.x
关键词
contamination; fin clip; fish body mucus; Neolamprologus pulcher
资金
- Biotechnology and Biological Sciences Research Council
- Natural Environment Research Council
- Royal Society University
- Natural Environment Research Council [NE/B50094X/1] Funding Source: researchfish
Non-destructive methods of collecting DNA from small fish species can be problematic, as fin clips can potentially affect behaviour or survivorship in the wild. Swabbing body mucus may provide a less invasive method of DNA collection. However, risk of contamination from other individuals in high density groups could give erroneous genotyping results. We compared multilocus microsatellite genotypes from the same individuals when collected at low and high density and compared this with fin clips. We found no differences between these categories, with a genotyping error rate of 0.42%, validating the use of body mucus swabbing for DNA collection in fish.
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