期刊
MOLECULAR ECOLOGY RESOURCES
卷 9, 期 1, 页码 4-18出版社
WILEY
DOI: 10.1111/j.1755-0998.2008.02469.x
关键词
bloodmeal; DNA barcoding; heteroduplex analysis; microsatellites; PCR; RFLP
资金
- American Society for Microbiology post-doctoral fellowship
- Centers for Disease Control and Prevention
- Robert E. Shope International Fellowship in Infectious Diseases
DNA-based methods have greatly enhanced the sensitivity and specificity of hematophagous arthropod bloodmeal identification. A variety of methods have been applied to study the blood-feeding behaviour of mosquitoes, ticks, black flies and other blood-feeding arthropods as it relates to host-parasite interactions and pathogen transmission. Overviews of the molecular techniques used for bloodmeal identification, their advantages, disadvantages and applications are presented for DNA sequencing, group-specific polymerase chain reaction primers, restriction fragment length polymorphism, real-time polymerase chain reaction, heteroduplex analysis, reverse line-blot hybridization and DNA profiling. Technical challenges to bloodmeal identification including digestion and analysis of mixed bloodmeals are discussed. Analysis of bloodmeal identification results remains a challenge to the field, particularly with regard to incorporation of vertebrate census and ecology data. Future research directions for molecular analysis of arthropod bloodmeals are proposed.
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