期刊
MOLECULAR ECOLOGY RESOURCES
卷 9, 期 3, 页码 934-937出版社
WILEY
DOI: 10.1111/j.1755-0998.2009.02517.x
关键词
Biological invasion; Insect; Invasive Species; Microsatellites; Nuclear marker
资金
- French ANR [ANR-06-BDIV-008-01]
A total of 18 microsatellite DNA loci were isolated and characterized from the harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae). We optimized a multiplex panel consisting of two polymerase chain reactions, allowing the genotyping of all loci. The number of alleles and heterozygosity observed at each locus ranged from 1 to 12 and from 0 to 100%, respectively. After Bonferroni correction for multiple tests, none of the loci deviated significantly from Hardy-Weinberg equilibrium and there was no indication of significant linkage disequilibrium among pairs of loci. Successful cross-species amplification was obtained for only three of the seven tested species of Coccinellidae.
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