期刊
MOLECULAR ECOLOGY RESOURCES
卷 8, 期 1, 页码 211-214出版社
WILEY
DOI: 10.1111/j.1471-8286.2007.01928.x
关键词
Arecaceae; CISP; conserved intron-scanning primers; intron; nuclear gene; Palmae
In this study, 96 nuclear 'conserved intron-scanning primers' were screened across subfamilies the Arecaceae (palms) for potential use in research focused on palm evolutionary biology. Primers were evaluated based on their ability to amplify single polymerase chain reaction products in Arecaceae, the clarity of sequencing reads, and the interspecific variability observed. Ultimately, the results suggest that: (i) seven of the loci are likely to be suitable when comparing non-Arecaceae outgroups and Arecaceae ingroups; (ii) seven loci may be of use when comparing subfamilies of Arecaceae; and (iii) four of the loci may be of use when comparing closely related genera.
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