期刊
MOLECULAR ECOLOGY
卷 21, 期 11, 页码 2565-2573出版社
WILEY
DOI: 10.1111/j.1365-294X.2011.05418.x
关键词
biological diversity; molecular detection; pyrosequencing; threatened species; wildlife conservation
资金
- University of Copenhagen
- Natural History Museum of Denmark
- Danish National Research Foundation
Freshwater ecosystems are among the most endangered habitats on Earth, with thousands of animal species known to be threatened or already extinct. Reliable monitoring of threatened organisms is crucial for data-driven conservation actions but remains a challenge owing to nonstandardized methods that depend on practical and taxonomic expertise, which is rapidly declining. Here, we show that a diversity of rare and threatened freshwater animalsrepresenting amphibians, fish, mammals, insects and crustaceanscan be detected and quantified based on DNA obtained directly from small water samples of lakes, ponds and streams. We successfully validate our findings in a controlled mesocosm experiment and show that DNA becomes undetectable within 2 weeks after removal of animals, indicating that DNA traces are near contemporary with presence of the species. We further demonstrate that entire faunas of amphibians and fish can be detected by high-throughput sequencing of DNA extracted from pond water. Our findings underpin the ubiquitous nature of DNA traces in the environment and establish environmental DNA as a tool for monitoring rare and threatened species across a wide range of taxonomic groups.
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