期刊
MOLECULAR CELL
卷 53, 期 4, 页码 591-605出版社
CELL PRESS
DOI: 10.1016/j.molcel.2014.01.019
关键词
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资金
- European Union
- Deutsche Forschungsgemeinschaft [1781/1-1]
Faithful chromosome segregation is mandatory for cell and organismal viability. Kinetochores, large protein assemblies embedded in centromeric chromatin, establish a mechanical link between chromosomes and spindle microtubules. The KMN network, a conserved 10-subunit kinetochore complex, harbors the microtubule-binding interface. RWD domains in the KMN subunits Spc24 and Spc25 mediate kinetochore targeting of the microtubule-binding subunits by interacting with the Mis12 complex, a KMN sub-complex that tethers directly onto the underlying chromatin layer. Here, we show that Knl1, aKMN sub-unit involved in mitotic checkpoint signaling, also contains RWD domains that bind the Mis12 complex and that mediate kinetochore targeting of Knl1. By reporting the first 3D electron microscopy structure of the KMN network, we provide a comprehensive framework to interpret how interactions of RWD-containing proteins with the Mis12 complex shape KMN network topology. Our observations unveil a regular pattern in the construction of the outer kinetochore.
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