期刊
MOLECULAR CELL
卷 56, 期 5, 页码 667-680出版社
CELL PRESS
DOI: 10.1016/j.molcel.2014.10.026
关键词
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资金
- CNRS
- Danish National Research Foundation
- Agence Nationale pour la Recherche (ANR) [ANR-08-Blan-0038-01, ANR-12-BSV8-0014-01]
- Fondation pour la Recherche Medicale (FRM)
- Fondation Bettencourt-Schueller
- National Institutes of Health
- Deutsche Forschungsgemeinschaft
- EMBO
- FRM
- Agence Nationale de la Recherche (ANR) [ANR-08-BLAN-0038, ANR-12-BSV8-0014] Funding Source: Agence Nationale de la Recherche (ANR)
Widely transcribed compact genomes must cope with the major challenge of frequent overlapping or concurrent transcription events. Efficient and timely transcription termination is crucial to control pervasive transcription and prevent transcriptional interference. In yeast, transcription termination of RNA polymerase II (RNAPII) occurs via two possible pathways that both require recognition of termination signals on nascent RNA by specific factors. We describe here an additional mechanism of transcription termination for RNAPII and demonstrate its biological significance. We show that the transcriptional activator Reb1p bound to DNA is a roadblock for RNAPII, which pauses and is ubiquitinated, thus triggering termination. Reb1p-dependent termination generates a class of cryptic transcripts that are degraded in the nucleus by the exosome. We also observed transcriptional interference between neighboring genes in the absence of Reb1p. This work demonstrates the importance of roadblock termination for controlling pervasive transcription and preventing transcription through gene regulatory regions.
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