期刊
MOLECULAR CELL
卷 50, 期 4, 页码 589-600出版社
CELL PRESS
DOI: 10.1016/j.molcel.2013.04.032
关键词
-
资金
- National Institutes of Health [GM041784]
- European Research Council under the European Union's Seventh Framework Programme (FP7)/ERC [242905]
- European Research Council (ERC) [242905] Funding Source: European Research Council (ERC)
Replication protein A (RPA) is an essential eukaryotic single-stranded DNA binding protein with a central role in DNA metabolism. RPA directly participates in DNA double-strand break repair by stimulating 5'-3' end resection by the Sgs1/BLM helicase and Dna2 endonuclease in vitro. Here we investigated the role of RPA in end resection in vivo, using a heat-inducible degron system that allows rapid conditional depletion of RPA in Saccharomyces cerevisiae. We found that RPA depletion eliminated both the Sgs1-Dna2-and Exo1-dependent extensive resection pathways and synergized with mre11 Delta to prevent end resection. The short single-stranded DNA tails formed in the absence of RPA were unstable due to 3' strand loss and the formation of fold-back hairpin structures that required resection initiation and Pol32-dependent DNA synthesis. Thus, RPA is required to generate ssDNA, and also to protect ssDNA from degradation and inappropriate annealing that could lead to genome rearrangements.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据