期刊
MOLECULAR CELL
卷 50, 期 6, 页码 869-881出版社
CELL PRESS
DOI: 10.1016/j.molcel.2013.05.007
关键词
-
资金
- Israel Science Foundation [ISF-Blkura 838/10]
- Israel Science Foundation (ISF) [ISF 61/09]
- ISF-Morasha [64/12]
- Israel Cancer Association
- Israel Cancer Research Foundation
- ISF [1015/10, 241/11]
- Marie Curie International Reintegration Grant
- Azrieli Foundation
- Edmond J. Safra Center for Bioinformatics at Tel Aviv University
The initial step in microRNA (miRNA) biogenesis requires processing of the precursor miRNA (pre-miRNA) from a longer primary transcript. Many pre-miRNAs originate from introns, and both a mature miRNA and a spliced RNA can be generated from the same transcription unit. We have identified a mechanism in which RNA splicing negatively regulates the processing of pre-miRNAs that overlap exon-intron junctions. Computational analysis identified dozens of such pre-miRNAs, and experimental validation demonstrated competitive interaction between the Microprocessor complex and the splicing machinery. Tissue-specific alternative splicing regulates maturation of one such miRNA, miR-412, resulting in effects on its targets that code a protein network involved in neuronal cell death processes. This mode of regulation specifically controls maturation of splice-site-overlapping pre-miRNAs but not pre-miRNAs located completely within introns or exons of the same transcript. Our data present a biological role of alternative splicing in regulation of miRNA biogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据