期刊
MOLECULAR CELL
卷 49, 期 1, 页码 43-54出版社
CELL PRESS
DOI: 10.1016/j.molcel.2012.11.008
关键词
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资金
- CNRS (Atip)
- Institut Curie
- Association pour la Recherche contre le Cancer
- Ligue Nationale contre le Cancer
- Electricite de France
- Fondation pour la Recherche Medicale
Meiotic chromosomes are organized into arrays of loops that are anchored to the chromosome axis structure. Programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination, catalyzed by Spoil and accessory DSB proteins, form in loop sequences in promoters, whereas the DSB proteins are located on chromosome axes. Mechanisms bridging these two chromosomal regions for DSB formation have remained elusive. Here we show that Spp1, a conserved member of the histone H3K4 methyltransferase Set1 complex, is required for normal levels of DSB formation and is associated with chromosome axes during meiosis, where it physically interacts with the Mer2 DSB protein. The PHD finger module of Spp1, which reads H3K4 methylation close to promoters, promotes DSB formation by tethering these regions to chromosome axes and activating cleavage by the DSB proteins. This paper provides the molecular mechanism linking DSB sequences to chromosome axes and explains why H3K4 methylation is important for meiotic recombination.
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