期刊
MOLECULAR CELL
卷 42, 期 6, 页码 806-816出版社
CELL PRESS
DOI: 10.1016/j.molcel.2011.04.012
关键词
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资金
- European Research Council [206117]
- Spanish Ministry of Science and Innovation [FIS2008-0025]
- Royal Society University
- BBSRC
- European Research Council (ERC) [206117] Funding Source: European Research Council (ERC)
AddAB is a helicase-nuclease that processes double-stranded DNA breaks for repair by homologous recombination. This process is modulated by Chi recombination hotspots: specific DNA sequences that attenuate the nuclease activity of the translocating AddAB complex to promote downstream recombination. Using a combination of kinetic and imaging techniques, we show that AddAB translocation is not coupled to DNA unwinding in the absence of single-stranded DNA binding proteins because nascent single-stranded DNA immediately re-anneals behind the moving enzyme. However, recognition of recombination hotspot sequences during translocation activates unwinding by coupling these activities, thereby ensuring the downstream formation of single-stranded DNA that is required for RecA-mediated recombinational repair. In addition to their implications for the mechanism of double-stranded DNA break repair, these observations may affect our implementation and interpretation of helicase assays and our understanding of helicase mechanisms in general.
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