期刊
MOLECULAR CELL
卷 43, 期 1, 页码 85-96出版社
CELL PRESS
DOI: 10.1016/j.molcel.2011.05.020
关键词
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资金
- Cancer Research UK
- EU [MRTN-CT-2004-005428]
- AIRC
The interplay between sequence-specific DNA-binding transcription factors, histone-modifying enzymes, and chromatin-remodeling enzymes underpins transcriptional regulation. Although it is known how single domains of chromatin readers bind specific histone modifications, how combinations of histone marks are recognized and decoded is poorly understood. Moreover, the role of histone binding in regulating the enzymatic activity of chromatin readers is not known. Here we focus on the TGF-beta superfamily transcriptional repressor TIF1 gamma/TRIM33/Ectodermin and demonstrate that its PHD finger-bromodomain constitutes a multivalent histone-binding module that specifically binds histone H3 tails unmethylated at K4 and R2 and acetylated at two key lysines. TIF1 gamma's ability to ubiquitinate its substrate Smad4 requires its PHD finger-bromodomain, as does its transcriptional repressor activity. Most importantly, TIF1 gamma's E3 ubiquitin ligase activity is induced by histone binding. We propose a model of TIF1 gamma activity in which it dictates the residence time of activated Smad complexes at promoters of TGF-beta superfamily target genes.
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