期刊
MOLECULAR CELL
卷 43, 期 6, 页码 1033-1039出版社
CELL PRESS
DOI: 10.1016/j.molcel.2011.07.030
关键词
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资金
- Spanish Ministry of Science [BIO2008-01091, 363]
- MC [510183]
- EURASNET [LSHG-CT-2005-518238]
- CSIC [2009201195]
- Agaur
- Carlos III Ph.D. fellowship
- ICREA Funding Source: Custom
Poor understanding of the spliceosomal mechanisms to select intronic 3' ends (3'ss) is a major obstacle to deciphering eukaryotic genomes. Here, we discern the rules for global 3'ss selection in yeast. We show that, in contrast to the uniformity of yeast splicing, the spliceosome uses all available 3'ss within a distance window from the intronic branch site (BS), and that in similar to 70% of all possible 3'ss this is likely to be mediated by pre-mRNA structures. Our results reveal that one of these RNA folds acts as an RNA thermosensor, modulating alternative splicing in response to heat shock by controlling alternate 3'ss availability. Thus, our data point to a deeper role for the pre-mRNA in the control of its own fate, and to a simple mechanism for some alternative splicing.
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