期刊
MOLECULAR CELL
卷 39, 期 2, 页码 292-299出版社
CELL PRESS
DOI: 10.1016/j.molcel.2010.05.015
关键词
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资金
- Japan Science and Technology Agency (JST)
- Japan Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- International Human Frontier Science Program Organization (HFSP)
- New Energy and Industrial Technology Development Organization (NEDO)
- JSPS
- Grants-in-Aid for Scientific Research [22380033] Funding Source: KAKEN
Small silencing RNAs-small interfering RNAs (siRNAs) or microRNAs (miRNAs)-direct posttranscriptional gene silencing of their mRNA targets as guides for the RNA-induced silencing complex (RISC). Both siRNAs and miRNAs are born double stranded. Surprisingly, loading these small RNA duplexes into Argonaute proteins, the core components of RISC, requires ATP, whereas separating the two small RNA strands within Argonaute does not. Here we show that the Hsc70/Hsp90 chaperone machinery is required to load small RNA duplexes into Argonaute proteins, but not for subsequent strand separation or target cleavage. We envision that the chaperone machinery uses ATP and mediates a conformational opening of Ago proteins so that they can receive bulky small RNA duplexes. Our data suggest that the chaperone machinery may serve as the driving force for the RISC assembly pathway.
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