期刊
MOLECULAR CELL
卷 36, 期 2, 页码 231-244出版社
CELL PRESS
DOI: 10.1016/j.molcel.2009.09.020
关键词
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资金
- Fundacao para Ciencia e Tecnologia (Lisboa, Portugal) [SFRH/BD/11803/2003, SFRH/BD/17629/2004/H6BM]
- NIH [DK074798]
- DRCRF [DRG-1983-08]
- Yeast Resource Center [R41 RR01 1823]
- NIGMS [GM63348, R01 GM58800]
- NSF [MCB-0618433]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/11803/2003, SFRH/BD/17629/2004] Funding Source: FCT
Endogenous small RNAs (endo-siRNAs) interact with Argonaute (AGO) proteins to mediate sequence-specific regulation of diverse biological processes. Here, we combine deep-sequencing and genetic approaches to explore the biogenesis and function of endo-siRNAs in C. elegans. We describe conditional alleles of the Dicer-related helicase, drh-3, that abrogate both RNA interference and the biogenesis of endo-siRNAs, called 22G-RNAs. DRH-3 is a core component of RNA-dependent RNA polymerase (RdRP) complexes essential for several distinct 22G-RNA systems. We show that, in the germline, one system is dependent on worm-specific AGOs, including WAGO-1, which localizes to germline nuage structures called P granules. WAGO-1 silences certain genes, transposons, pseudogenes, and cryptic loci. Finally, we demonstrate that components of the nonsense-mediated decay pathway function in at least one WAGO-mediated surveillance pathway. These findings broaden our understanding of the biogenesis and diversity of 22G-RNAs and suggest additional regulatory functions for small RNAs.
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