期刊
MOLECULAR CELL
卷 30, 期 5, 页码 649-656出版社
CELL PRESS
DOI: 10.1016/j.molcel.2008.04.016
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资金
- NHLBI NIH HHS [T32 HL007089] Funding Source: Medline
- NIGMS NIH HHS [R01 GM073180-02, R01 GM073180-01, R01 GM073180-03, R01 GM073180, R01 GM073180-04] Funding Source: Medline
Cell differentiation requires the ability to detect and respond appropriately to a variety of extracellular signals. Here we investigate a differentiation switch induced by changes in the concentration of a single stimulus. Yeast cells exposed to high doses of mating pheromone undergo cell division arrest. Cells at intermediate doses become elongated and divide in the direction of a pheromone gradient (chemotropic growth). Either of the pheromone-responsive MAP kinases, Fus3 and Kss1, promotes cell elongation, but only Fus3 promotes chemotropic growth. Whereas Kss1 is activated rapidly and with a graded dose-response profile, Fus3 is activated slowly and exhibits a steeper dose-response relationship (ultra-sensitivity). Fus3 activity requires the scaffold protein Ste5; when binding to Ste5 is abrogated, Fus3 behaves like Kss1, and the cells no longer respond to a gradient or mate efficiently with distant partners. We propose that scaffold proteins serve to modulate the temporal and dose-response behavior of the MAP kinase.
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