期刊
MOLECULAR CELL
卷 32, 期 2, 页码 190-197出版社
CELL PRESS
DOI: 10.1016/j.molcel.2008.10.001
关键词
-
资金
- Howard Hughes Medical Institute
- National Insitutes of Health (NIH) [R01 GM55440, R37 GM29169, R01 GM059425]
A crucial step in translation is the translocation of tRNAs through the ribosome. In the transition from one canonical site to the other, the tRNAs acquire intermediate configurations, so-called hybrid states. At this stage, the small subunit is rotated with respect to the large subunit, and the anticodon stem loops reside in the A and P sites of the small subunit, while the acceptor ends interact with the P and E sites of the large subunit. In this work, by means of cryo-EM and particle classification procedures, we visualize the hybrid state of both A/P and P/E tRNAs in an authentic factor-free ribosome complex during translocation. In addition, we show how the repositioning of the tRNAs goes hand in hand with the change in the interplay between S13, L1 stalk, L5, H68, H69, and H38 that is caused by the ratcheting of the small subunit.
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