期刊
MOLECULAR CANCER RESEARCH
卷 7, 期 8, 页码 1328-1341出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1541-7786.MCR-08-0548
关键词
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资金
- Ministero Istruzione [12-25201001-14]
- Universita e Ricerca, Ricerca Corrente [2.30]
- Ministero della Salute, Italy [RF 06-81]
Here, we show that NF-kappa B-HIF-1 interaction contributed to breast cancer metastatic capacity by means of an incomplete epithelial/mesenchymal transition and influencing migration, as shown in 1833 (human) and 4T1 (mouse) metastatic cells after different stimuli. The 1833 and the transforming growth factor-beta 1-exposed 4T1 cells showed both epithelial (E-cadherins) and mesenchymal (N-cadherins and vimentin) markers, and common mechanisms contributed to the retention of certain epithelial characteristics and the control of migration. The complex NF-kappa B-HIF-1 reciprocal regulation and the enhanced c-Jun expression played a functional role in exacerbating the invasiveness of 1833 cells after p50/p65 transfection and of 4T1 cells exposed to transforming growth factor-beta 1. Twist expression seemed to exert a permissive role also regulating epithelial/mesenchymal transition markers. After c-Src wild-type (Srcwt) transfection, c-Src-signal transducer overexpression in 1833 cells increased HIF-1 transactivating activity and invasiveness, and changed E-cadherin/N-cadherin ratio versus mesenchymal phenotype. The transcription factor pattern and the motile phenotype of metastatic 1833 cells were influenced by p65-lysine acetylation and HDAC-dependent epigenetic mechanisms, which positively regulated basal NF-kappa B and HIF-1 activities. However, HDAC3 acted as a corepressor of NF-kappa B activity in parental MDA-MB231 cells, thus explaining many differences from the derived 1833 clone, including reduced HIF-1 alpha and c-Jun expression. Invasiveness was differently affected by HDAC knockdown in 1833 and MDA-MB231 cells. We suggest that acetylation/deacetylation are critical in establishing the bone-metastatic gene signature of 1833 cells by regulating the activity of NF-kappa B and HIF-1, and further clarity the epigenetic control of transcription factor network in the motile phenotype of 1833 cells. (Mol Cancer Res 2009;7(8):1328-41)
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