期刊
MOLECULAR CANCER
卷 12, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1476-4598-12-111
关键词
Secreted phospholipase; A(2); Breast cancer; Cell survival; Apoptosis; Lipid droplets; Fatty acid oxidation; Lipid signaling; Etomoxir; Carnitine palmitoyltransferase 1; AMP-activated protein kinase
资金
- Slovenian Research Agency [P1-0207, J3-0386]
- French-Slovene partnership project [BI-FR/12-13-PROTEUS-006]
- French Government Scholarsh
- CNRS
- Association for International Cancer Research
- Fondation ARC pour la recherche sur le cancer
Background: Alterations in lipid metabolism are inherent to the metabolic transformations that support tumorigenesis. The relationship between the synthesis, storage and use of lipids and their importance in cancer is poorly understood. The human group X secreted phospholipase A(2) (hGX sPLA(2)) releases fatty acids (FAs) from cell membranes and lipoproteins, but its involvement in the regulation of cellular FA metabolism and cancer is not known. Results: Here we demonstrate that hGX sPLA(2) induces lipid droplet (LD) formation in invasive breast cancer cells, stimulates their proliferation and prevents their death on serum deprivation. The effects of hGX sPLA2 are shown to be dependent on its enzymatic activity, are mimicked by oleic acid and include activation of protein kinase B/Akt, a cell survival signaling kinase. The hGX sPLA(2)-stimulated LD biogenesis is accompanied by AMP-activated protein kinase (AMPK) activation, up-regulation of FA oxidation enzymes and the LD-coating protein perilipin 2, and suppression of lipogenic gene expression. Prolonged activation of AMPK inhibited hGX sPLA(2)-induced LD formation, while etomoxir, an inhibitor of FA oxidation, abrogated both LD formation and cell survival. The hGX sPLA(2)-induced changes in lipid metabolism provide a minimal immediate proliferative advantage during growth under optimal conditions, but they confer to the breast cancer cells a sustained ability to resist apoptosis during nutrient and growth factor limitation. Conclusion: Our results identify hGX sPLA(2) as a novel modulator of lipid metabolism that promotes breast cancer cell growth and survival by stimulating LD formation and FA oxidation.
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