期刊
MOLECULAR BREEDING
卷 33, 期 3, 页码 519-529出版社
SPRINGER
DOI: 10.1007/s11032-013-9968-z
关键词
Late maturity alpha-amylase (LMA); High pI alpha-amylase; alpha-Amy-1 genes; Triticum aestivum; Wheat; Quantitative real-time RT-PCR
资金
- University of Adelaide
- Australian Grains Research and Development Corporation
- South Australian Research and Development Institute
Late maturity alpha-amylase (LMA) is a genetic defect involving the synthesis of high pI isozymes of alpha-amylase encoded by alpha-Amy-1 genes during the later stages of grain development. The aims of this investigation were to determine both the number of expressed alpha-Amy-1 genes and their relative transcript abundance. Sub-cloning and sequencing of expressed high pI alpha-amylase genes in developing wheat seeds revealed three insertion/deletion patterns in the 3' untranslated region and numerous single nucleotide polymorphisms at the 3' end of alpha-Amy-1. The genetic variations defined 36 alpha-Amy-1 gene sequences that were expressed on the onset of LMA in doubled haploid progenies (SpM25, SpM52 and SpM127) derived from the cross Spica (LMA)/Maringa (non-LMA). Five isoelectric point groups were predicted based on the translated partial coding sequences. The potential application of quantitative real-time RT-PCR in screening wheat genotypes for LMA is discussed.
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