期刊
MOLECULAR BREEDING
卷 31, 期 3, 页码 537-548出版社
SPRINGER
DOI: 10.1007/s11032-012-9811-y
关键词
Capsaicin synthase; Capsicum; Pungency; Pun1; Putative aminotransferase
资金
- Vegetable Breeding Research Center through R&D Convergence Center Support Program, Ministry for Food, Agriculture, Forestry and Fisheries [710001-03]
- Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center), Rural Development Administration, Republic of Korea [PJ00818701]
Pungency in pepper (Capsicum annuum L.) has unique characteristics due to the alkaloid compound group, capsaicinoids, which includes capsaicin. Although capsaicinoids have been proved to have pharmacological and physiological effects on human health, the application of capsaicinoids has been limited because of their pungency. Capsinoids found in non-pungent peppers share closely related structures with capsaicinoids and show similar biological effects. Previous studies demonstrated that mutations in the p-AMT gene were related to the production of capsinoids; however, the pathway of capsinoid synthesis has not yet been fully elucidated. In this study, we performed genetic analysis to determine the mechanism of capsinoid synthesis using a F6 recombinant inbred line population. In this population, the presence/absence of capsinoids co-segregated with the genotype of the Pun1 locus, without exception. In addition, we screened the patterns of capsinoid synthesis and the correlation between the Pun1 locus and capsinoid synthesis in p-AMT mutant accessions. In Capsicum germplasms, we selected amino-acid-substituted mutants in the PLP binding domain of the p-AMT gene. Capsinoids were not synthesized with the recessive pun1 gene, regardless of the p-AMT genotype, and no relationship was found between p-AMT mutant type and capsinoid content. We concluded that the Pun1 gene, which is responsible for capsaicinoid synthesis, also controls capsinoid synthesis.
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