期刊
MOLECULAR BREEDING
卷 31, 期 3, 页码 737-741出版社
SPRINGER
DOI: 10.1007/s11032-012-9812-x
关键词
Lycopene-epsilon-cyclase; Phytoene synthase; Lycopene-beta-cyclase; Flour b* colour; Lutein; Wheat
Flour b* colour is an important grain quality parameter for specific wheat end-products. The genetic control of b* colour in Australian wheat accessions is controlled by quantitative trait loci (QTL) on chromosomes 3A, 3B, 7A and 7B accumulating lutein, a compound of the carotenoid biosynthetic pathway. The relationship between lutein accumulation and flour b* colour provides an opportunity to identify sequence variants of genes encoding enzymes from the biosynthetic pathway that may control trait variation. This study identified a single nucleotide polymorphism (SNP) in the gene encoding lycopene-epsilon-cylcase on chromosome 3A (e-LYC3A) between two wheat accessions Ajana and WAWHT2074, identifying two alleles, e-LYC3Aa and e-LYC3Ab, respectively. e-LCY3Ab was present in 62.5 % of the wheat accessions analysed. A highly significant (P < 0.01) association with QTL on chromosome 3A in two mapping populations indicated that e-LYC3A is functionally associated with b* colour variation in some Australian wheat accessions. The SNP induced a serine/glycine substitution at amino acid residue 123 and a subtle change in protein folding at amino acid residue 119. The e-LYC3A SNP may be considered along with other alleles and genes on homoeologous group 3 and 7 chromosomes for selecting desirable flour b* colour variation in marker-assisted breeding.
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