期刊
MOLECULAR BIOTECHNOLOGY
卷 53, 期 3, 页码 289-299出版社
HUMANA PRESS INC
DOI: 10.1007/s12033-012-9526-7
关键词
Extracellular secretion; beta-Glucosidase; Glucose inhibition insensitive; Proteomics; Transglycosylation; Trichoderma citrinoviride
资金
- CSIR under NWP-09 network program
beta-Glucosidases are of pivotal importance in bioconversion of carbonic biomass into fermentable and other useful metabolites, food industry, biotransformation, glyco-trimming of metabolome, etc. Trichoderma citrinoviride when grown on delignified Lantana camara produced a beta-glucosidase and secreted it out in the medium. The extracellularly secreted beta-glucosidase of T. citrinoviride was homogeneity purified and then characterized for its kinetic properties and proteomic characteristics. The 90 kDa enzyme was monomeric in nature, optimally active at pH 5.5 and the catalytic reaction rate was highest at 55A degrees C. Uniquely, the enzyme was insensitive to inhibition by glucose (up to 5 mM). It also possessed catalytic ability of transglycosylation, as it could catalyze conversion of geraniol into its glucoside. MALDI-TOF assisted proteomic analysis revealed its high degree of sequence similarity with family 3 glycoside hydrolases.
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