期刊
MOLECULAR BIOTECHNOLOGY
卷 40, 期 3, 页码 261-268出版社
HUMANA PRESS INC
DOI: 10.1007/s12033-008-9083-2
关键词
Cholera toxin B subunit; Rice; Endosperm-specific promoter; Plant-based vaccine
资金
- International Cooperation Program of the Ministry of Science and Technology, Republic of Korea
- Bilateral Intergovernmental Science & Technology Cooperation, KOR [13/99]
- Hungarian Scientific Research Fund [OTKA T 034791]
- ICGEB [CRP/HUN00-02]
The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1. The recombinant vector was transformed into rice plants using a biolistic-mediated transformation method. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis. A high level of CTB (2.1% of total soluble protein) was expressed in the endosperm tissue of the transgenic rice plants. The synthetic CTB produced only in the rice endosperm demonstrated strong affinity for G(M1)-ganglioside, thereby suggesting that the CTB subunits formed an active pentamer. The successful expression of CTB genes in transgenic plants makes it a powerful tool for the development of a plant-derived edible vaccine.
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