期刊
MOLECULAR BIOSYSTEMS
卷 10, 期 11, 页码 2838-2849出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4mb00403e
关键词
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资金
- National Science Foundation [MCB-1052557]
- Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy [DE-FG-0397ER20259]
- G. Thomas and Anita Hargrove Center for Plant Genomic Research
- Major Research Instrumentation grant from the National Science Foundation [DBI-1229749]
- NSF [MCB-1052557]
- DOE
- NSF MRI
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1052557] Funding Source: National Science Foundation
Podophyllum hexandrum and, to a much lesser extent P. peltatum, are sources of podophyllotoxin, extensively used as a chemical scaffold for various anti-cancer drugs. In this study, integrated omics technologies (including advanced mass spectrometry/metabolomics, transcriptome sequencing/gene assemblies, and bioinformatics) gave unequivocal evidence that both plant species possess a hitherto unknown aporphine alkaloid metabolic pathway. Specifically, RNA-seq transcriptome sequencing and bioinformatics guided gene assemblies/analyses in silico suggested presence of transcripts homologous to genes encoding all known steps in aporphine alkaloid biosynthesis. A comprehensive metabolomics analysis, including UPLC-TOF-MS and MALDI-MS imaging in situ, then enabled detection, identification, localization and quantification of the aporphine alkaloids, magnoflorine, corytuberine and muricinine, in the underground and aerial tissues. Interestingly, the purported presence of alkaloids in Podophyllum species has been enigmatic since the 19th century, remaining unresolved until now. The evolutionary and phylogenetic ramifications of this discovery are discussed.
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