期刊
AQUACULTURE NUTRITION
卷 22, 期 3, 页码 631-642出版社
WILEY
DOI: 10.1111/anu.12277
关键词
digestive enzymes; gut histology; gut microbiota; rearing temperature; short-chain fructooligosaccharides; turbot
类别
资金
- European Regional Development Fund (ERDF) through the COMPETE - Operational Competitiveness Programme
- FCT - Foundation for Science and Technology [PEst-C/MAR/LA0015/2011]
- Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BD/76139/2011, BPD/39688/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/76139/2011] Funding Source: FCT
The gut microbiomes of fish play important roles in host development, digestion and health. Evidence suggests that abiotic factors, such as diet and rearing temperature, could affect fish gut microbiota. In this study, the effect of dietary shortchain fructooligosaccharides (scFOS) on turbot intestinal health, microbiota and digestive enzymes was investigated at two rearing temperatures: 15 and 20 degrees C. Four practical diets were supplemented with scFOS at 0, 5, 10 and 20 g kg(-1). scFOS did not affect fish performance. PCR-DGGE did not show differences in bacterial profiles between dietary treatments; however, the number of operational taxonomic units, richness and diversity were higher at 20 degrees C. Enzyme activities in the foregut were not affected by rearing temperature, while in the hindgut, enzyme activities were higher at 15 degrees C. Total alkaline protease, alpha-amylase and lipase activities in the foregut were higher in fish fed 20 g kg(-1) scFOS. Prebiotic supplementation had no effect on hindgut alpha-amylase activity, while lipase activity of fish reared at 20 degrees C was higher in fish fed diet with 5 g kg(-1) scFOS. No differences were observed in intestinal morphology. This was the first study to simultaneously evaluate the effect of dietary prebiotic level and rearing temperature on fish intestinal microbiota and health.
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