期刊
MOLECULAR BIOSYSTEMS
卷 6, 期 12, 页码 2417-2422出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0mb00024h
关键词
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资金
- S973 project [2007CB914100, 2010CB912704, 2009CB825607]
- 863 project [2006AA02A308]
- NSF of China [20735005, 20875016]
- Shanghai project [07JC14003, 08DZ2293601, B109]
A novel one-pipeline approach is reported, which can demonstrate glycoprotein identification and obtain intact glycosylation information after glycopeptide-level enrichment, without de-glycosylation. The proposed workflow has two enrichment steps plus two proteolytic processes: enriched glycoproteins were digested to peptides by Lys-C, and then enriched again and secondly digested by trypsin. In the resulting mixture, with a reasonable complexity, intact glycopeptides could be preserved and utilized informatively for glycosylation analysis, and non-glycopeptides for protein identification. In both standard protein mixture tests and real sample analysis, the resulting glycopeptides and non-glycopeptides were proved to play their expected roles, thus more confident protein glycosylation information was obtained.
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