期刊
MOLECULAR BIOSYSTEMS
卷 6, 期 6, 页码 958-966出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b927494d
关键词
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资金
- Vanderbilt University College of Arts and Science
- Vanderbilt Institute of Chemical Biology
- Vanderbilt Institute of Integrative Biosystems Research and Education
- US Defense Threat Reduction Agency [HDTRA-09-1-0013]
- Waters Inc
- NIH [NS41740, GM54544]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM054544] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS041740] Funding Source: NIH RePORTER
Phosphatidylethanolamine (PtdEtn) is one of the most abundant phospholipids in many animal cell types. The Drosophila easily shocked (eas(2)) mutant, used as an epilepsy model, is null for the PtdEtn biosynthetic enzyme, ethanolamine kinase. This mutant displays bang sensitive paralysis, and was previously shown to have decreased levels of PtdEtn. We have developed a highly selective and sensitive measurement strategy using ion mobility-mass spectrometry for the relative quantitation of intact phospholipid species directly from isolated brain tissue of eas mutants. Over 1200 distinct lipid signals are observed and within this population 38, including PtdEtn, phosphatidylinositol (PtdIns) and phosphatidylcholine (PtdCho) species are identified to have changed significantly (p < 0.03) between mutant and control tissue. This method has revealed for the first time the structural complexity and biosynthetic interconnectedness of specific PtdEtn and PtdIns lipid species within tissue, and provides great molecular detail compared to traditionally used detection techniques.
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