期刊
MOLECULAR BIOLOGY REPORTS
卷 40, 期 10, 页码 5781-5789出版社
SPRINGER
DOI: 10.1007/s11033-013-2681-5
关键词
CdTe Quantum dots; Papain; Thermodynamic parameters; Conformational change; Interactions
资金
- National Natural Science Foundation of China [21203035, 21261005]
- National Science Fund for Distinguished Young Scholars of China [21225313]
- Scientific Research Fund of Guangxi Provincial Education Department [2013YB138]
- Guangxi Natural Science Foundation [2013GXNSFCA019005, 2013GXNSFBA019029]
Fluorescent quantum dots (QDs) have been widely applied in biological and biomedical areas, but relatively little is known about the interaction of QDs with some natural enzymes. Herein, the interactions between 3-mercaptopropionic acid-capped CdTe QDs (MPA-QDs) and papain were systematically investigated by UV-Vis absorption spectra, fluorescence spectra and circular dichroism (CD) spectra under the physiological conditions. The fluorescence spectra results indicated that MPA-QDs quenched the fluorescence intensity of papain. The modified Stern-Volmer quenching constant K (a) at different temperatures and the corresponding thermodynamic parameters Delta H, Delta G and Delta S were also calculated. The binding of MPA-QDs and papain is a result of the formation of QDs-papain complex and the electrostatic interactions play a major role in stabilizing the complex. The CD technique was further used to analyze the conformational changes of papain induced by MPA-QDs and the results indicated that the biological activity of papain was affected by MPA-QDs dramatically.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据