期刊
MOLECULAR BIOLOGY REPORTS
卷 40, 期 1, 页码 73-80出版社
SPRINGER
DOI: 10.1007/s11033-012-2014-0
关键词
Landrace and Korean native pigs; Fatty acid composition; Single nucleotide polymorphism; Association; Stearoyl-CoA desaturase
资金
- Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea [110061-03-1-CG000]
Fatty acid (FA) composition is one of the most important parameters for the evaluation of meat quality. The stearoyl-CoA desaturase (SCD) gene is considered a positional candidate gene affecting FA composition in SSC14, based on previous quantitative trait loci studies. To evaluate the association of the SCD gene with FA composition in a Korean native pig x Landrace F-2 intercross population, we genotyped six single nucleotide polymorphisms (SNPs) of the SCD gene located in promoter region [2 SNPs (g.-353T > C, g.-233T > C)], exonic region [1 SNP (g.817C > T) in exon 2] and 3' UTR [3 SNPs (g.13311C > G, g.14384G > A, and g.14424C > T)] identified by massively parallel sequencing technology. Eighteen FA composition traits were measured in more than the 950 F-2 animals. A mixed-effect model was used to evaluate associations between these SNPs and FA composition traits in the F-2 intercross population. A detailed investigation detected that the five FA composition traits [palmitoleic acid (C16:1), stearic acid (C18:0), arachidic acid (C20:0), saturated FA, and unsaturated FA] were highly significant (P < 4.7 x 10(-5); C20:0) in association with the SNP g.-233T > C, SNP g.817C > T, SNP g.13311C > G and SNP g.14384G > A in the SCD gene, whereas SNP g.14424C > T was only significantly associated with palmitoleic acid (C16:1, P = 1.4 x 10(-3)). No significant association of FA composition traits with SNP g.-353T > C was detected. In particular, the SNP g.14384G > A accounted for 30.6 % of the additive genetic variance of palmitoleic acid (P = 1.9 x 10(-10)). These results suggest the SCD gene has a strong effect on FA composition in the crossbred pig population.
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