期刊
MOLECULAR BIOLOGY REPORTS
卷 40, 期 5, 页码 3549-3555出版社
SPRINGER
DOI: 10.1007/s11033-012-2428-8
关键词
Polyphenol oxidases (PPOs); Silkworm, Bombyx mori; Molting hormone; RNA interference; Metamorphosis
资金
- National Basic Research Program of China [2012CB114601]
- National Natural Science Foundation of China [30972141/C120110]
- Key Project of Zhejiang Government [2011C14006]
- Science and Technology Innovation Team of Zhejiang Province [2010R50031]
- Chinese Universities Scientific Fund
Insect molting is an important developmental process of metamorphosis, which is initiated by molting hormone. The molting process includes the activation of dermal cells, epidermal cells separation, molting fluid secretion, the formation of new epidermis and old epidermis excoriation etc. Polyphenol oxidases (PPOs), dopa decarboxylase and acetyltransferase are necessary enzymes for this process. Traditionally, the phenol oxidase was considered as an enzyme for epidermal layer's tanning and melanization. This work suggested that polyphenol oxidases are one set of the key enzymes in molting, which closely related with the role of ecdysone in regulation of molting processes. The data showed that the expression peak of phenol oxidase in silkworm is higher during molting stage, and decreases after molting. The significant increase in the ecdysone levels of haemolymph was observed in the artificially fed silkworm larvae with ecdysone hormone. Consistently, the phenol oxidase expression was significantly elevated compared to the control. PPO1 RNAi induced phenol oxidase expression obviously declined in the silkworm larvae, and caused the pupae incomplete pupation. Overall, the results described that the phenol oxidase expression is regulated by the molting hormone, and is a necessary enzyme for the silkworm molting.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据