期刊
MOLECULAR BIOLOGY REPORTS
卷 39, 期 9, 页码 9233-9238出版社
SPRINGER
DOI: 10.1007/s11033-012-1796-4
关键词
Purification; Antarctic; Pseudoalteromonas sp.; Sea ice; Cold-active lipase
资金
- National Natural Science Foundation of China [31100037]
- Natural Science Foundation of Shandong Province [ZR2009DQ023, ZR2011CM003]
- Program of Excellent Team in the Harbin Institute of Technology
- China Postdoctoral Science Foundation [20090451004]
- Heilongjiang Province Postdoctoral Science Foundation [LBH-Z09176]
- Natural Scientific Research Innovation Foundation in Harbin Institute of Technology [HIT-NSRIF201011]
An extracellular cold-active lipase from Antarctic sea ice bacteria Pseudoalteromonas sp. NJ 70 was purified and characterized. The overall purification based on lipase activity was 27.5-fold with a yield of 25.4 %. The purified lipase showed as a single band on SDS-PAGE with an apparent molecular weight of 37 kDa. The optimum temperature and pH were 35 A degrees C and 7.0, respectively. The lipase activity was enhanced by Ca2+ and Mg2+, while was partially inhibited by other metals such as Cu2+, Zn2+, Ba2+, Pb2+, Fe2+ and Mn2+. The lipase had high tolerance to a wide range of NaCl concentrations (0-2 M NaCl). It exhibited high levels of activity in the presence of DTT, Thiourea, H2O2 as well as in the presence of various detergents such as Span 60, Tween-80, Triton X-100. In addition, the lipase showed a preference for long-chain p-nitrophenyl esters (C-12-C-18). These results indicated that this lipase could be a novel cold-active lipase.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据