期刊
MOLECULAR BIOLOGY REPORTS
卷 40, 期 3, 页码 2231-2241出版社
SPRINGER
DOI: 10.1007/s11033-012-2286-4
关键词
cDNA-amplified fragment length polymorphism; Polygonum minus; Methyl jasmonate; Salicylic acid; Stress-related gene; Secondary metabolite
资金
- Universiti Kebangsaan Malaysia through University Research Grants [UKM-AP-BPB-14-2009, UKM-GUP-KPB-08-33-135]
- National Science Fellowship (NSF)
- Ministry of Science, Technology and Innovation in Malaysia
- Rural Development Administration (RDA), Suwon, Korea
The importance of plant secondary metabolites for both mankind and the plant itself has long been established. However, despite extensive research on plant secondary metabolites, plant secondary metabolism and its regulation still remained poorly characterized. In this present study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) transcript profiling was applied to generate the expression profiles of Polygonum minus in response to salicylic acid (SA) and methyl jasmonate (MeJA) elicitations. This study reveals two different sets of genes induced by SA and MeJA, respectively where stress-related genes were proved to lead to the expression of genes involved in plant secondary metabolite biosynthetic pathways. A total of 98 transcript-derived fragments (TDFs) were up-regulated, including 46 from SA-treated and 52 from MeJA-treated samples. The cDNA-AFLP transcripts generated using 64 different Mse1/Taq1 primer combinations showed that treatments with SA and MeJA induced genes mostly involved in scavenging reactive oxygen species, including zeaxanthin epoxidase, cytosolic ascorbate peroxidase 1 and peroxidase. Of these stress-related genes, 15 % of other annotated TDFs are involved mainly in secondary metabolic processes where among these, two genes encoding (+)-delta cadinene synthase and cinnamoyl-CoA reductase were highlighted.
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