期刊
MOLECULAR BIOLOGY REPORTS
卷 39, 期 5, 页码 5115-5123出版社
SPRINGER
DOI: 10.1007/s11033-011-1307-z
关键词
Binding site; Circular dichroism; Fluorescence spectrum; Human serum albumin; Molecular modeling; 10-Hydroxycamptothecin
资金
- National Natural Science Foundation of China [20921062]
- Hubei Province Department of Education [D20101302]
- National Innovation Experiment Program for University Students [101048902]
In this work, fluorescence spectroscopy in combination with circular dichroism spectroscopy and molecular modeling was employed to investigate the binding of 10-hydroxycamptothecin (HCPT) to human serum albumin (HSA) under simulative physiological conditions. The experiment results showed that the fluorescence quenching of HSA by HCPT was a result of the formation of HCPT-HSA complex. The corresponding association constants (K (a)) between HCPT and HSA at four different temperatures were determined according to the modified Stern-Volmer equation. The results of thermodynamic parameters Delta G, Delta H, and Delta S indicated that hydrogen bonds and van der Waals forces played major roles for HCPT-HSA association. Site marker competitive displacement experiment indicated that the binding of HCPT to HSA primarily took place in sub-domain IIA (site I). Molecular docking study further confirmed the binding mode and the binding site obtained by fluorescence and site marker competitive experiments. The conformational investigation showed that the presence of HCPT decreased the alpha-helical content of HSA and induced the slight unfolding of the polypeptides of protein, which confirmed some micro-environmental and conformational changes of HSA molecules.
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