期刊
MOLECULAR BIOLOGY REPORTS
卷 39, 期 3, 页码 2375-2383出版社
SPRINGER
DOI: 10.1007/s11033-011-0988-7
关键词
Western Ghats of India; DNA barcoding; Butterflies; Nymphalidae; Cytochrome c oxidase I
资金
- DBT (Department of Biotechnology), Govt. of India [No-BT/PR8351/NDB/51/138/2006]
- DBT
We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.
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