期刊
MOLECULAR BIOLOGY REPORTS
卷 37, 期 7, 页码 3171-3176出版社
SPRINGER
DOI: 10.1007/s11033-009-9897-4
关键词
MicroRNA; Bombyx mori; Computational prediction; Target gene
资金
- National High Tech Development Project of China
- 863 Program [2007AA100504]
- National Basic Research 973 Program [2005CB121004]
MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate expression of genes at post-transcriptional levels. Increasing evidence has shown that miRNAs play multiple roles in biological processes, including development, cell proliferation and apoptosis. Based on the conservation of miRNAs sequence, using a computational homology search based on genomic survey sequence analysis, a total of 16 novel miRNAs were identified and characteristics such as family and evolutionary conservation have been described. By using these newly identified miRNAs, the mRNA database of silkworm was blasted and 21 potential targets of miRNAs were detected. Most of these miRNA targeted genes were predicted to encode transcription factors. The semi-stem loop RT-PCR based assays were performed and found that silkworm miRNAs have diverse expression patterns during development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据